• BALB/c mice were treated with 1 µg/mouse IL-12 (solid square) or vehicle control (VC; open triangle) for 4 consecutive days from day 0, as indicated with arrows, then leukocyte populations analyzed at various days post treatment.
  • Peripheral lymph node cells were pooled from axillary, inguinal, brachial, and cervical lymph nodes (excluding mesenteric (mes) LN). Where explicitly stated in the figure legends mesLN were collected. After lymphoid organ disaggregation, cells were washed and resuspended in complete media (RPMI-1640, 5%FCS, 2 mM L-gln, 100 U/ml penicillin/streptomycin, and 2. 5×10–5 M 2 Beta-ME. Anti-CD3 (145-2C11, BD) at 0-1 µg/ml and anti-CD28 (37. 51, BD) at 2 µg/ml mAb were coated on plates (1h, 37°C, in PBS) and washed twice. In transient TCR stimulations, cells were moved from Ab-coated to fresh wells on d2. aAPC were described previously
  • The black bars show the production levels from IL-18 and IL-12 co-stimulated Th1 cells, grey bars show those from IL-18 solely stimulated Th1 cells, and the white bars show those from no secondary stimulated Th1 cells.
  • Naive OT-1 T cells were purified using a CD8 isolation kit with supplemental anti-CD44, and labeled with 5 Mu M CFSE. T cell and celltype NA >NK cell depleted splenocytes were purified by a similar procedure as OT-1 T cells with an antibody mixture containing biotin-Alpha TCR Beta (H57-597) , biotin-Alpha TCR Gamma Delta (eBioGL3) and biotin-Alpha celltype NA >NK1. 1 (pk136) and used as APCs. APCs were pulsed with 0. 2–5ng/ml peptide (SIINFEKL and all variant peptides were from Genemed Synthesis) and 1µg/ml LPS at 37°C for 1 hour followed by extensive washing. OT-1 T cells and APCs were mixed and cultured in the presence of 2. 5ng/ml hTGF-Beta 1 (&systems). As indicated, 1000U/ml IFN-Alpha A (PBL InterferonSource) , 20ng/ml IFN-Gamma and 20ng/ml IL-12 were added to the culture. 50U/ml IL-2 (eBioscience) was added one day later. 10µg/ml Alpha TGF-Beta neutralizing antibody (clone#1D11, R&D systems) was added as indicated in the absence exogenous hTGF-Beta 1. To calculate the T cell number during…
  • Splenic cells (2 × 106/mL) from age 4 to 6 weeks na i ve NOD. BDC2. 5. FoxP3GFP. DTR mice were stimulated with p79 peptide (0. 5 Mu mol/L) under Th1 or Th17 conditions for 4 days. For polarization into Th1 cells, the stimulation was carried out in the presence of recombinant (r) IL-12 (10 ng/mL) and anti–IL-4 (10 Mu g/mL [11B11]). For Th17 polarization, the culture was supplemented with recombinant transforming growth factor-Beta (3 ng/mL) , rIL-6 (20 ng/mL) , anti–IFN-Gamma (10 Mu g/mL [4–6A4]) , and anti–IL-4 (10 Mu g/mL [11B11]) antibodies and rIL-23 (20 ng/mL& ) (only for the last 2 days).

Mouse Interleukin-12 p40 protein (Recombinant) (STJP000548)

SKU:
STJP000548

£136.50 - £228.50
Current Stock:
Host: CHO cells
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Mouse Interleukin-12 p40-protein was developed from cho cells. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered PBS solution.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA
Immunoreactivity: The ED (50) determined by the dose-dependent stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is <.1 ng/ml, corresponding to a specific activity of > x units/mg. NA
Gene Symbol: Il12b
Gene ID: 16160
Uniprot ID: IL12B_MOUSE
Immunogen Region: Mature chain
Immunogen: Optimized DNA sequence encoding mouse Interleukin-12 mature chain was over-expressed in CHo cells. NA
Immunogen Sequence: MCPQKLTISW FAIVLLVSPL MAMWELEKDV YVVEVDWTPD APGETVNLTC DTPEEDDITW TSDQRHGVIG SGKTLTITVK EFLDAGQYTC HKGGETLSHS HLLLHKKENG IWSTEILKNF KNKTFLKCEA PNYSGRFTCS WLVQRNMDLK FNIKSSSSSP DSRAVTCGMA SLSAEKVTLD QRDYEKYSVS CQEDVTCPTA EETLPIELAL EARQQNKYEN YSTSFFIR
Function Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Protein Name Interleukin-12 Subunit Beta
Il-12b
Cytotoxic Lymphocyte Maturation Factor 40 Kda Subunit
Clmf P40
Il-12 Subunit P40
Cellular Localisation Secreted
Alternative Protein Names Interleukin-12 Subunit Beta protein
Il-12b protein
Cytotoxic Lymphocyte Maturation Factor 40 Kda Subunit protein
Clmf P40 protein
Il-12 Subunit P40 protein
Il12b protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance