![Splenic cells (2 × 106/mL) from age 4 to 6 weeks na i ve NOD. BDC2. 5. FoxP3GFP. DTR mice were stimulated with p79 peptide (0. 5 Mu mol/L) under Th1 or Th17 conditions for 4 days. For polarization into Th1 cells, the stimulation was carried out in the presence of recombinant (r) IL-12 (10 ng/mL) and anti–IL-4 (10 Mu g/mL [11B11]). For Th17 polarization, the culture was supplemented with recombinant transforming growth factor-Beta (3 ng/mL) , rIL-6 (20 ng/mL) , anti–IFN-Gamma (10 Mu g/mL [4–6A4]) , and anti–IL-4 (10 Mu g/mL [11B11]) antibodies and rIL-23 (20 ng/mL& ) (only for the last 2 days).](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/151289/345280/STJP000548_6__05505.1679324593.jpg?c=1 "Splenic cells (2 × 106/mL) from age 4 to 6 weeks na i ve NOD. BDC2. 5. FoxP3GFP. DTR mice were stimulated with p79 peptide (0. 5 Mu mol/L) under Th1 or Th17 conditions for 4 days. For polarization into Th1 cells, the stimulation was carried out in the presence of recombinant (r) IL-12 (10 ng/mL) and anti–IL-4 (10 Mu g/mL [11B11]). For Th17 polarization, the culture was supplemented with recombinant transforming growth factor-Beta (3 ng/mL) , rIL-6 (20 ng/mL) , anti–IFN-Gamma (10 Mu g/mL [4–6A4]) , and anti–IL-4 (10 Mu g/mL [11B11]) antibodies and rIL-23 (20 ng/mL& ) (only for the last 2 days).")
| Host: | CHO cells |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Mouse Interleukin-12 p40-protein was developed from cho cells. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 Mu m filtered PBS solution. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The ED (50) determined by the dose-dependent stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is <.1 ng/ml, corresponding to a specific activity of > x units/mg. NA |
| Gene Symbol: | Il12b |
| Gene ID: | 16160 |
| Uniprot ID: | IL12B_MOUSE |
| Immunogen Region: | Mature chain |
| Immunogen: | Optimized DNA sequence encoding mouse Interleukin-12 mature chain was over-expressed in CHo cells. NA |
| Immunogen Sequence: | MCPQKLTISW FAIVLLVSPL MAMWELEKDV YVVEVDWTPD APGETVNLTC DTPEEDDITW TSDQRHGVIG SGKTLTITVK EFLDAGQYTC HKGGETLSHS HLLLHKKENG IWSTEILKNF KNKTFLKCEA PNYSGRFTCS WLVQRNMDLK FNIKSSSSSP DSRAVTCGMA SLSAEKVTLD QRDYEKYSVS CQEDVTCPTA EETLPIELAL EARQQNKYEN YSTSFFIR |
| Function | Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. |
| Protein Name | Interleukin-12 Subunit BetaIl-12bCytotoxic Lymphocyte Maturation Factor 40 Kda SubunitClmf P40Il-12 Subunit P40 |
| Database Links | Reactome: R-MMU-9020591Reactome: -MMU-9020933 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-12 Subunit Beta proteinIl-12b proteinCytotoxic Lymphocyte Maturation Factor 40 Kda Subunit proteinClmf P40 proteinIl-12 Subunit P40 proteinIl12b protein |
Information sourced from Uniprot.org