| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.01 EU/Mu g |
| Immunoreactivity: | Measured in a cell proliferation assay using murine 2E8 cells. The ED50 for this effect is 1.1-4.2 ng/mL, corresponding to a specific activity of 2.38×105~9.1×105 units/mg. |
| Gene Symbol: | Il7 |
| Gene ID: | 16196 |
| Uniprot ID: | IL7_MOUSE |
| Immunogen Region: | Glu26-Ile154 |
| Immunogen: | Recombinant Mouse IL-7 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu26-Ile154) of mouse IL-7 (Accession #NP_032397.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | ECHIKDKEGKAYESVLMISI DELDKMTGTDSNCPNNEPNF FRKHVCDDTKEAAFLNRAAR KLKQFLKMNISEEFNVHLLT VSQGTQTLVNCTSKEEKNVK EQKKNDACFLKRLLREIKTC WNKILKGSI |
| Function | Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5. |
| Protein Name | Interleukin-7Il-7 |
| Database Links | Reactome: R-MMU-1266695 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-7 proteinIl-7 proteinIl7 proteinIl-7 protein |
Information sourced from Uniprot.org