| Host: |
E.coli |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 0.1 EU/Mu g |
| Immunoreactivity: |
Measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50 for this effect is 29.1-116.4 pg/mL, corresponding to a specific activity of 8.59×106~3.44×107 units/mg. |
| Immunogen Region: |
Met23-Ser335 & Ala21-Ala196 |
| Immunogen: |
Recombinant Mouse IL-23/IL-12B&IL-23A Protein is produced by HEK293 expression system. The target protein is expressed with sequence (N-His (IL-23a) &No tag (IL-12b) ) of mouse Met23-Ser335＆Ala21-Ala196 (Accession #) fused with a 6×His tag at the N-t |
| Immunogen Sequence: |
VPIHKVQDDTKTLIKTIVTR INDISHTQSVSARQRVTGLD FIPGLHPILSLSKMDQTLAV YQQILTSLPSQNVLQIAHDL ENLRDLLHLLAFSKSCSLPQ TRGLQKPESLDGVLEASLYS TEVVALSRLQGSLQDILQQL DLSPEC |
| Background | Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T?cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T?cells. While IL-12 acts on both na?ve and memory human Tnbsp;cells, the effects of IL-23 is restricted to memory Tcells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8-and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
