| Host: |
HEK293 cells |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse IL-2-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Mouse IL-2 at 2 Mu g/mL (100 Mu L/well) can bind Mouse IL-2 with a linear range of 0.1-43.8 ng/mL.2. Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this |
| Gene Symbol: |
Il2 |
| Gene ID: |
16183 |
| Uniprot ID: |
IL2_MOUSE |
| Immunogen: |
Recombinant Mouse IL-2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala21-Gln169) of mouse IL2 (Accession #NP_032392.1) fused with a 6×His tag at the C-terminus. |
| Function | Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells. |
| Protein Name | Interleukin-2Il-2T-Cell Growth FactorTcgf |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-2 proteinIl-2 proteinT-Cell Growth Factor proteinTcgf proteinIl2 proteinIl-2 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
