| Host: |
Rat/Rabbit |
| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: |
This IL-18 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Conjugation: |
Unconjugated/Biotin |
| Formulation: |
Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Purification: |
Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Concentration: |
0.5 mg/mL |
| Storage Instruction: |
Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Determination Method: |
Colormetric |
| Gene Symbol: |
Il18 |
| Gene ID: |
16173 |
| Uniprot ID: |
IL18_MOUSE |
| Immunogen: |
Capture immunogen: Recombinant Mouse IL-18 protien.Detection Immunogen: Recombinant Mouse IL-18 protien. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. |
| Protein Name | Interleukin-18Il-18Interferon Gamma-Inducing FactorIfn-Gamma-Inducing FactorInterleukin-1 GammaIl-1 Gamma |
| Database Links | Reactome: R-MMU-448706Reactome: -MMU-5620971Reactome: -MMU-9012546 |
| Cellular Localisation | CytoplasmSecretedThe Precursor Is CytosolicIn Response To Inflammasome-Activating SignalsCleaved And SecretedMature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) PoreIn ContrastThe Precursor Form Is Not ReleasedDue To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During MaturationThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative Antibody Pair Names | Interleukin-18 antibody pairIl-18 antibody pairInterferon Gamma-Inducing Factor antibody pairIfn-Gamma-Inducing Factor antibody pairInterleukin-1 Gamma antibody pairIl-1 Gamma antibody pairIl18 antibody pairIgif antibody pair |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
