Mouse IL-12 protein (Recombinant) (STJP000547)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenDNA sequence encoding extracellular domain of mouse IL-12A fused to a C-terminal polyHis tag and aDNA sequence encoding extracellular domain of mouse IL-12Bfused to a C-terminal polyHis tagwere co-expressed in HEK cells. NA
General Information
| Short Description | Recombinant-Mouse IL-12-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution pH7.4. |
| Storage Instruction | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The activity was determined by its ability to bind recombinant Mouse IL-12RB in functional ELISA. NA |
Target Information
| Gene Symbol | Il12a |
| Gene ID | 16159 |
| Uniprot ID | IL12A_MOUSE |
| Immunogen | DNA sequence encoding extracellular domain of mouse IL-12A fused to a C-terminal polyHis tag and aDNA sequence encoding extracellular domain of mouse IL-12Bfused to a C-terminal polyHis tagwere co-expressed in HEK cells. NA |
| Immunogen Region | ECD |
| Immunogen Sequence | MCQSRYLLFL ATLALLNHLS LARVIPVSGP ARCLSQSRNL LKTTDDMVKT AREKLKHYSC TAEDIDHEDI TRDQTSTLKT CLPLELHKNE SCLATRETSS TTRGSCLPPQ KTSLMMTLCL GSIYEDLKMY QTEFQAINAA LQNHNHQQII LDKGMLVAID ELMQSLNHNG ETLRQKPPVG EADPYRVKMK LCILLHAFST RVVTINRVMG YLSSA MCPQKLTISW FA |
Additional Info
| Function | Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity. Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes. As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and also functions as an immune-suppressive cytokine. Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers. Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites. |
| Protein Name | Interleukin-12 Subunit AlphaIl-12aCytotoxic Lymphocyte Maturation Factor 35 Kda SubunitClmf P35Il-12 Subunit P35 |
| Database Links | Reactome: R-MMU-8984722Reactome: -MMU-9020591 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-12 Subunit Alpha proteinIl-12a proteinCytotoxic Lymphocyte Maturation Factor 35 Kda Subunit proteinClmf P35 proteinIl-12 Subunit P35 proteinIl12a protein |
Information sourced from Uniprot.org