Mouse IL-12 protein (Recombinant) (STJP000547)

SKU:
STJP000547

Shipping:
Free Shipping
Current Stock:
Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Mouse IL-12-protein was developed from hek293. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered PBS solution pH7.4.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA
Immunoreactivity: The activity was determined by its ability to bind recombinant Mouse IL-12RB in functional ELISA. NA
Gene Symbol: Il12a
Gene ID: 16159
Uniprot ID: IL12A_MOUSE
Immunogen Region: ECD
Immunogen: DNA sequence encoding extracellular domain of mouse IL-12A fused to a C-terminal polyHis tag and aDNA sequence encoding extracellular domain of mouse IL-12Bfused to a C-terminal polyHis tagwere co-expressed in HEK cells. NA
Immunogen Sequence: MCQSRYLLFL ATLALLNHLS LARVIPVSGP ARCLSQSRNL LKTTDDMVKT AREKLKHYSC TAEDIDHEDI TRDQTSTLKT CLPLELHKNE SCLATRETSS TTRGSCLPPQ KTSLMMTLCL GSIYEDLKMY QTEFQAINAA LQNHNHQQII LDKGMLVAID ELMQSLNHNG ETLRQKPPVG EADPYRVKMK LCILLHAFST RVVTINRVMG YLSSA MCPQKLTISW FA
Function Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity. Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes. As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and functions also as an immune-suppressive cytokine. Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers. Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites.
Protein Name Interleukin-12 Subunit Alpha
Il-12a
Cytotoxic Lymphocyte Maturation Factor 35 Kda Subunit
Clmf P35
Il-12 Subunit P35
Cellular Localisation Secreted
Alternative Protein Names Interleukin-12 Subunit Alpha protein
Il-12a protein
Cytotoxic Lymphocyte Maturation Factor 35 Kda Subunit protein
Clmf P35 protein
Il-12 Subunit P35 protein
Il12a protein

Information sourced from Uniprot.org