| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.054ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This IkBKb Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma, tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
Ikbkb |
| Gene ID: |
16150 |
| Uniprot ID: |
IKKB_MOUSE |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Detected in heart (at protein level). Expressed in liver, kidney and spleen. |
| Post Translational Modifications | Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ.which enhances activity. Phosphorylated by MAP3K7/TAK1 in response to NOD1 and NOD2 signaling, promoting activation and phosphorylation of NF-kappa-B inhibitors, leading to NF-kappa-B activation. Once activated, autophosphorylates on the C-terminal serine cluster.which decreases activity and prevents prolonged activation of the inflammatory response. Phosphorylated by the IKK-related kinases TBK1 and IKBKE, which is associated with reduced CHUK/IKKA and IKBKB activity and NF-kappa-B-dependent gene transcription. Dephosphorylated at Ser-177 and Ser-181 by PPM1A and PPM1B. Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. 'Ser-163' may not serve as a monoubiquitination site. Ubiquitination on 'Ser-163' may modulate phosphorylation on C-terminal serine residues. Hydroxylated by PHD1/EGLN2, loss of hydroxylation under hypoxic conditions results in activation of NF-kappa-B. |
| Function | Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as a part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NAA10, NCOA3, BCL10 and IRS1. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus. Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylates STAT1 at 'Thr-748' which restricts interferon signaling and anti-inflammatory responses and promotes innate inflammatory responses. IKBKB-mediated phosphorylation of STAT1 at 'Thr-748' promotes binding of STAT1 to the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6. It also promotes binding of STAT1 to the IL12B promoter and activation of IL12B transcription. |
| Protein Name | Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit BetaI-Kappa-B-Kinase BetaIkk-BIkk-BetaIkbkbI-Kappa-B Kinase 2Ikk2Nuclear Factor Nf-Kappa-B Inhibitor Kinase BetaNfkbikbSerine/Threonine Protein Kinase Ikbkb |
| Database Links | Reactome: R-MMU-1169091Reactome: -MMU-1810476Reactome: -MMU-202424Reactome: -MMU-209543Reactome: -MMU-209560Reactome: -MMU-2871837Reactome: -MMU-445989Reactome: -MMU-5357905Reactome: -MMU-5357956Reactome: -MMU-5607764Reactome: -MMU-5684264Reactome: -MMU-9020702Reactome: -MMU-933542Reactome: -MMU-937039Reactome: -MMU-937041Reactome: -MMU-975144Reactome: -MMU-9758274Reactome: -MMU-9833482Reactome: -MMU-9860276 |
| Cellular Localisation | CytoplasmNucleusMembrane RaftColocalized With Dpp4 In Membrane Rafts |
| Alternative ELISA Names | Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Beta ELISA kitI-Kappa-B-Kinase Beta ELISA kitIkk-B ELISA kitIkk-Beta ELISA kitIkbkb ELISA kitI-Kappa-B Kinase 2 ELISA kitIkk2 ELISA kitNuclear Factor Nf-Kappa-B Inhibitor Kinase Beta ELISA kitNfkbikb ELISA kitSerine/Threonine Protein Kinase Ikbkb ELISA kitIkbkb ELISA kitIkkb ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
