Mouse IGFBP-2 (Insulin-Like Growth Factor-Binding Protein 2) Antibody Pair (STJAP00536)
General Information
| Short Description | This IGFBP-2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | Igfbp2 |
| Gene ID | 16008 |
| Uniprot ID | IBP2_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse IGFBP-2 protien.Detection Immunogen: Recombinant Mouse IGFBP-2 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Cleaved by MMP9 leading to release of free IGF2 from IGFBP2-IGF2 complex, which contributes to enhance the motility and the growth of astrocytes. O-glycosylated. |
| Function | Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Functions coordinately with receptor protein tyrosine phosphatase beta/PTPRB and the IGF1 receptor to regulate IGF1-mediated signaling by stimulating the phosphorylation of PTEN leading to its inactivation and AKT1 activation. Plays a positive role in cell migration via interaction with integrin alpha5/ITGA5 through an RGD motif. Additionally, interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells. Upon mitochondrial damage, facilitates apoptosis with ITGA5 of podocytes, and then activates the phosphorylation of focal adhesion kinase (FAK)-mediated mitochondrial injury. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 2Ibp-2Igf-Binding Protein 2Igfbp-2Migfbp-2 |
| Database Links | Reactome: R-MMU-381426 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Insulin-Like Growth Factor-Binding Protein 2 antibody pairIbp-2 antibody pairIgf-Binding Protein 2 antibody pairIgfbp-2 antibody pairMigfbp-2 antibody pairIgfbp2 antibody pairIgfbp-2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse IGFBP-2 (Insulin-Like Growth Factor-Binding Protein 2) Antibody Pair (STJAP00536)
This antibody pair detects mouse IGFBP-2 (Insulin-Like Growth Factor-Binding Protein 2), corresponding to UniProt entry P47877. Reactivity has been confirmed for mouse samples only.
The capture antibody is unconjugated and rabbit-hosted, purified by Protein A or G, while the detection antibody is biotin-conjugated and goat-hosted, purified by antigen affinity. Both components are supplied at 0.5 mg/mL.
This pair has been validated for use in ELISA. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
This antibody pair is suitable for use with serum, plasma, and other biological fluids from mouse origin.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.