Mouse IgE protein (Recombinant) (C-His) (STJP003112)
SPECIFICATIONS
HostMouse
ConjugationUnconjugated
ImmunogenRecombinant Mouse IgE Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Pro50-Lys388) of mouse IgE (Accession #) fused with and 6ΓHis tag at the C-terminus.
General Information
| Short Description | Recombinant-Mouse IgE-C-His protein was developed in mouse using the region Pro50-Lys388. For use in research applications. |
| Host | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20Β°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Mouse IgE at 1 Mu g/mL (100 Mu L/well) can bind Mouse IgE Rabbit mAb with a linear range of 0.06-0.45 ng/mL. |
| Determination Method | < 0.1 EU/Mu g |
Target Information
| Gene Symbol | IGHEUniprot ID=IGHE_MOUSE" |
| Immunogen | Recombinant Mouse IgE Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Pro50-Lys388) of mouse IgE (Accession #) fused with and 6ΓHis tag at the C-terminus. |
| Immunogen Region | Pro50-Lys388 |
| Immunogen Sequence | PALGSELKVTTSQVTSWGKS AKNFTCHVTHPPSFNESRTI LVRPVNITEPTLELLHSSCD PNAFHSTIQLYCFIYGHILN DVSVSWLMDDREITDTLAQT VLIKEEGKLASTCSKLNITE QQWMSESTFTCKVTSQGVDY LAHTRRCPDHEPRGVITYLI PPSPLDLYQNGAPKLTCLVV DLESEKNVNVTWNQEKKTSV SASQWYTKHHNNATTSITSI LPVVAKDWIEGYGYQCIVD |
Additional Info
| Function | Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen. |
| Protein Name | Immunoglobulin Heavy Constant EpsilonIg Epsilon Chain C Region |
| Cellular Localisation | SecretedCell Membrane |
| Alternative Protein Names | Immunoglobulin Heavy Constant Epsilon proteinIg Epsilon Chain C Region proteinIGHE protein |
Information sourced from Uniprot.org