Mouse ICAM-1/CD54 (intercellular adhesion molecule 1) Sandwich ELISA Kit (STJE0019734)

SKU:
STJE0019734
£163.50 - £360.50
Free Shipping
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25-2000pg/mL
Short Description : This ICAM-1/CD54 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Icam1
Gene ID: 15894
Uniprot ID: ICAM1_MOUSE
Specificity: This kit recognizes Mouse ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Mouse ICAM-1/CD54 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
Protein Name Intercellular Adhesion Molecule 1
Icam-1
Mala-2
Myd10
Cd Antigen Cd54
Database Links Reactome: R-MMU-198933
Reactome: -MMU-216083
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names Intercellular Adhesion Molecule 1 ELISA kit
Icam-1 ELISA kit
Mala-2 ELISA kit
Myd10 ELISA kit
Cd Antigen Cd54 ELISA kit
Icam1 ELISA kit
Icam-1 ELISA kit
Specificity This kit recognizes Mouse ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Mouse ICAM-1/CD54 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 93
EDTA plasma (n=8) 95-107 101
Cell culture media (n=8) 94-108 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 103.43 196.42 853.47 96.67 202.08 925.10
Standard deviation 6.68 8.78 40.37 6.24 11.88 41.54
CV (%) 6.46 4.47 4.73 6.46 5.88 4.49

Related Products

Model Name Host Clonality Reactivity Application
STJE0006535 Rat ICAM-1/CD54 (Intercellular Adhesion Molecule 1) Sandwich ELISA Kit (STJE0006535) Rat ELISA
STJE0019990 Human ICAM-1 (intercellular adhesion molecule 1) Sandwich ELISA Kit (STJE0019990) Human ELISA
STJAP00528 Mouse ICAM-1/CD54 (Intercellular Adhesion Molecule 1) Antibody Pair (STJAP00528) Rat/Goat Mouse ELISA
STJAP00089 Rat ICAM-1/CD54 (Intercellular Adhesion Molecule 1) Antibody Pair (STJAP00089) Mouse/Goat Monoclonal/Polyclonal Rat ELISA
!