| Post Translational Modifications | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. |
| Protein Name | Intercellular Adhesion Molecule 1Icam-1Mala-2Myd10Cd Antigen Cd54 |
| Database Links | Reactome: R-MMU-198933Reactome: -MMU-216083 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative ELISA Names | Intercellular Adhesion Molecule 1 ELISA kitIcam-1 ELISA kitMala-2 ELISA kitMyd10 ELISA kitCd Antigen Cd54 ELISA kitIcam1 ELISA kitIcam-1 ELISA kit |
| Specificity | This kit recognizes Mouse ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Mouse ICAM-1/CD54 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-100 | 93 |
| EDTA plasma (n=8) | 95-107 | 101 |
| Cell culture media (n=8) | 94-108 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 103.43 | 196.42 | 853.47 | 96.67 | 202.08 | 925.10 |
| Standard deviation | 6.68 | 8.78 | 40.37 | 6.24 | 11.88 | 41.54 |
| CV (%) | 6.46 | 4.47 | 4.73 | 6.46 | 5.88 | 4.49 |
