| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 29.3pg/mL |
| Detection Limit: | 78.1-5000pg/mL |
| Short Description: | This HSD3b1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | Hsd3b1 |
| Gene ID: | 15492 |
| Uniprot ID: | 3BHS1_MOUSE |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Steroidogenic tissues (includes testes, ovaries and adrenal glands). |
| Post Translational Modifications | |
| Function | A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3-beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity. |
| Protein Name | 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I 3-Beta-Hsd I 3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase 3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase 3-Beta-Hydroxysteroid 3-Dehydrogenase Delta-5-3-Ketosteroid Isomerase Dihydrotestosterone Oxidoreductase Steroid Delta-Isomerase |
| Database Links | Reactome: R-MMU-193048 Reactome: -MMU-193993 Reactome: -MMU-194002 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Membrane Protein Mitochondrion Membrane |
| Alternative ELISA Names | 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1 ELISA kit 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I ELISA kit 3-Beta-Hsd I ELISA kit 3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase ELISA kit 3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase ELISA kit 3-Beta-Hydroxysteroid 3-Dehydrogenase ELISA kit Delta-5-3-Ketosteroid Isomerase ELISA kit Dihydrotestosterone Oxidoreductase ELISA kit Steroid Delta-Isomerase ELISA kit Hsd3b1 ELISA kit Hsd3b ELISA kit |
| output |
Information sourced from Uniprot.org
