Mouse HB-EGF (Heparin-binding Epidermal Growth Factor-like Growth Factor) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This mouse HB-EGF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Mouse |
| Sensitivity | 4.69pg/mL |
| Detection Limit | 7.81~500pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | Hbegf |
| Gene ID | 15200 |
| Uniprot ID | HBEGF_MOUSE |
| Specificity | This kit recognizes Mouse HB-EGF in samples. No significant cross-reactivity or interference between Mouse HB-EGF and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Most abundant in kidney, skeletal muscle, lung, spleen, brain and heart. |
| Post Translational Modifications | O-glycosylated. |
| Function | Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. |
| Protein Name | Proheparin-Binding Egf-Like Growth Factor Cleaved Into - Heparin-Binding Egf-Like Growth FactorHb-EgfHbegf |
| Database Links | Reactome: R-MMU-1227986Reactome: -MMU-1236394Reactome: -MMU-1250196Reactome: -MMU-1250342Reactome: -MMU-1250347Reactome: -MMU-1257604Reactome: -MMU-177929Reactome: -MMU-179812Reactome: -MMU-180292Reactome: -MMU-180336Reactome: -MMU-182971Reactome: -MMU-1963640Reactome: -MMU-1963642Reactome: -MMU-212718Reactome: -MMU-2179392Reactome: -MMU-5673001Reactome: -MMU-6785631Reactome: -MMU-6811558Reactome: -MMU-8847993Reactome: -MMU-8856825Reactome: -MMU-8856828Reactome: -MMU-8857538Reactome: -MMU-8863795Reactome: -MMU-9009391 |
| Cellular Localisation | Heparin-Binding Egf-Like Growth Factor: SecretedExtracellular SpaceMature Hb-Egf Is Released Into The Extracellular Space And Probably Binds To A ReceptorProheparin-Binding Egf-Like Growth Factor: Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative CLIA Names | Proheparin-Binding Egf-Like Growth Factor Cleaved Into - Heparin-Binding Egf-Like Growth Factor CLIA kitHb-Egf CLIA kitHbegf CLIA kitHbegf CLIA kitDtr CLIA kitHegfl CLIA kit |
| Specificity | This kit recognizes Mouse HB-EGF in samples. No significant cross-reactivity or interference between Mouse HB-EGF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 102-114 | 108 |
| EDTA plasma(n=8) | 100-115 | 106 |
| Cell culture media(n=8) | 94-106 | 100 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 26.55 | 43.56 | 186.52 | 26.51 | 46.06 | 197.33 |
| Standard deviation | 2.56 | 3.86 | 20.42 | 2.90 | 4.09 | 20.76 |
| CV (%) | 9.64 | 8.86 | 10.95 | 10.94 | 8.88 | 10.52 |