Mouse GRP58/PDIA3 protein (Recombinant) (His-Tag) (STJP019338)
SPECIFICATIONS
HostE.coli
ImmunogenMouse
General Information
| Short Description | Recombinant-Mouse GRP58/PDIA3-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, and 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity | Specific activity is >10 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reduction of insulin. |
Target Information
| Gene Symbol | Pdia3 |
| Gene ID | 14827 |
| Uniprot ID | PDIA3_MOUSE |
| Accession Number | NP_031978 |
| Immunogen | Mouse |
| Immunogen Region | 25-505aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | In caput and cauda epididymal spermatozoa, detected in the acrosome and principal piece (at protein level). |
| Post Translational Modifications | Phosphorylated. Within the major histocompatibility complex class I (MHC I) peptide loading complex forms reversible disulfide-linked heterodimers with TAPBP as part of its protein folding chaperone activity. This is essential to assist the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. |
| Function | Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone. Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity. |
| Protein Name | Protein Disulfide-Isomerase A358 Kda Glucose-Regulated Protein58 Kda Microsomal ProteinP58Disulfide Isomerase Er-60Endoplasmic Reticulum Resident Protein 57Er Protein 57Erp57Endoplasmic Reticulum Resident Protein 60Er Protein 60Erp60 |
| Database Links | Reactome: R-MMU-1236974Reactome: -MMU-901042Reactome: -MMU-983170 |
| Cellular Localisation | Endoplasmic ReticulumEndoplasmic Reticulum LumenMelanosome |
| Alternative Protein Names | Protein Disulfide-Isomerase A3 protein58 Kda Glucose-Regulated Protein protein58 Kda Microsomal Protein proteinP58 proteinDisulfide Isomerase Er-60 proteinEndoplasmic Reticulum Resident Protein 57 proteinEr Protein 57 proteinErp57 proteinEndoplasmic Reticulum Resident Protein 60 proteinEr Protein 60 proteinErp60 proteinPdia3 proteinErp proteinErp60 proteinGrp58 protein |
Information sourced from Uniprot.org