Mouse GFRAL/GFR alpha-like protein (Recombinant) (N-His-Avi) {Biotin} (STJP001652)
SPECIFICATIONS
HostHEK293
ConjugationBiotin
ImmunogenDNA sequence encoding Mouse GFRAL/GFR alpha-like including a N-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
General Information
| Short Description | Recombinant-Mouse GFRAL/GFR alpha-like-N-His-Avi protein was developed from hek293 and has a target region of N-His-Avi. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Biotin |
| Dilution Range | Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Formulation | Lyophilised from 0.2 ยตm filtered PBS solution pH7.4. |
| Storage Instruction | The lyophilized protein is stable for at least 1 year from date of receipt at-20ยฐC. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ยตg (1EU/ยตg). |
Target Information
| Gene Symbol | Gfral |
| Gene ID | 404194 |
| Uniprot ID | GFRAL_MOUSE |
| Immunogen | DNA sequence encoding Mouse GFRAL/GFR alpha-like including a N-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Immunogen Region | Gln20-Glu350 |
Additional Info
| Post Translational Modifications | Cleaved and inactivated by MMP14, inhibiting the GDF15-GFRAL aversive response. |
| Function | Brainstem-restricted receptor for GDF15 hormone, which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses. The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future. The GDF15-GFRAL aversive response is triggered by stresses, such as anticancer drugs (camptothecin or cisplatin), cancers or drugs such as metformin. Upon interaction with its ligand, GDF15, mediates the GDF15-induced autophosphorylation and activation of the RET tyrosine kinase receptor, leading to activation of MAPK- and AKT- signaling pathways. Ligand-binding activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues. |
| Protein Name | Gdnf Family Receptor Alpha-Like |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Membrane ProteinExtracellular Side |
| Alternative Protein Names | Gdnf Family Receptor Alpha-Like proteinGfral proteinGral protein |
Information sourced from Uniprot.org