| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.2 Mu m filtered solution of 20 mM Tris-HCl, pH 7.5, 50 mM Na2SO4, 0.5 mM DTT, 1 mM EDTA. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: |
Fgf1 |
| Gene ID: |
14164 |
| Uniprot ID: |
FGF1_MOUSE |
| Immunogen Region: |
Phe16-Asp155 |
| Immunogen: |
Recombinant Mouse FGF-1/aFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Phe16-Asp155) of mouse FGF1/FGFa/FGF acidic (Accession #P61148). |
| Immunogen Sequence: |
MWELEKDVYVVEVDWTPDAP GETVNLTCDTPEEDDITWTS DQRHGVIGSGKTLTITVKEF LDAGQYTCHKGGETLSHSHL LLHKKENGIWSTEILKNFKN KTFLKCEAPNYSGRFTCSWL VQRNMDLKFNIKSSSSSPDS RAVTCGMASLSAEKVTLDQR DYEKYSVSCQEDVTCPTAEE TLPIELALEARQQNKYENYS TSFFIRDIIKPDPPKNLQMK PLKNSQVEVSWEYPDSWST |
| Post Translational Modifications | In the nucleus, phosphorylated by PKC/PRKCD. |
| Function | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis. |
| Protein Name | Fibroblast Growth Factor 1Fgf-1Acidic Fibroblast Growth FactorAfgfHeparin-Binding Growth Factor 1Hbgf-1 |
| Database Links | Reactome: R-MMU-109704Reactome: -MMU-1257604Reactome: -MMU-190322Reactome: -MMU-190370Reactome: -MMU-190371Reactome: -MMU-190372Reactome: -MMU-190373Reactome: -MMU-190375Reactome: -MMU-190377Reactome: -MMU-5654219Reactome: -MMU-5654221Reactome: -MMU-5654227Reactome: -MMU-5654228Reactome: -MMU-5654687Reactome: -MMU-5654688Reactome: -MMU-5654689Reactome: -MMU-5654693Reactome: -MMU-5654695Reactome: -MMU-5654699Reactome: -MMU-5654700Reactome: -MMU-5654704Reactome: -MMU-5654706Reactome: -MMU-5654710Reactome: -MMU-5654712Reactome: -MMU-5654719Reactome: -MMU-5654720Reactome: -MMU-5654726Reactome: -MMU-5654727Reactome: -MMU-5654732Reactome: -MMU-5654733Reactome: -MMU-5673001Reactome: -MMU-6811558 |
| Cellular Localisation | SecretedCytoplasmCell CortexCytosolNucleusLacks A Cleavable Signal SequenceWithin The CytoplasmIt Is Transported To The Cell Membrane And Then Secreted By A Non-Classical Pathway That Requires Cu(2+) Ions And S100a13Secreted In A Complex With Syt1Binding Of Exogenous Fgf1 To Fgfr Facilitates Endocytosis Followed By Translocation Of Fgf1 Across Endosomal Membrane Into The CytosolNuclear Import From The Cytosol Requires The Classical Nuclear Import MachineryInvolving Proteins Kpna1 And Kpnb1As Well As Lrrc59 |
| Alternative Protein Names | Fibroblast Growth Factor 1 proteinFgf-1 proteinAcidic Fibroblast Growth Factor proteinAfgf proteinHeparin-Binding Growth Factor 1 proteinHbgf-1 proteinFgf1 proteinFgf-1 proteinFgfa protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
