| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-mouse EPO receptor (EPOR)-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
0.2 Mu m filtered PBS solution, pH7.2. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Gene Symbol: |
Epor |
| Gene ID: |
13857 |
| Uniprot ID: |
EPOR_MOUSE |
| Immunogen Region: |
ECD |
| Immunogen: |
Optimized DNA sequence encoding extracellular domain of mouse erythropoietin receptor including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Expressed in relatively mature erythroid progenitor cells and in EPO-responsive erythroleukemia cells. |
| Post Translational Modifications | The identity of the C-linked hexose on the WXXW motif has not been determined. It is probably mannose. On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-453 is required for PTPN6 interaction, Tyr-425 for PTPN11. Tyr-425 is also required for SOCS3 binding, but Tyr-453/Tyr-455 motif is the preferred binding site. Ubiquitinated by NOSIP.appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells. Ubiquitination at Lys-280 mediates receptor internalization, whereas ubiquitination at Lys-452 promotes trafficking of activated receptors to the lysosomes for degradation. |
| Function | Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase. |
| Protein Name | Erythropoietin ReceptorEpo-R |
| Database Links | Reactome: R-MMU-9006335Reactome: -MMU-9027276Reactome: -MMU-9027284 |
| Cellular Localisation | Isoform Epor-F: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Epor-S: Secreted |
| Alternative Protein Names | Erythropoietin Receptor proteinEpo-R proteinEpor protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
