| Host: |
HEK293 cells |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse EPO-R/Epor-C-6His protein was developed from hek293 cells and has a target region of C-6His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Epor |
| Gene ID: |
13857 |
| Uniprot ID: |
EPOR_MOUSE |
| Immunogen Region: |
Ala25-Pro249 |
| Immunogen: |
Recombinant Mouse EPO-R/Epor Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala25-Pro249) of mouse EPO-R/Epor (Accession #NP_034279.3) fused with and a 6×His tag at the C-terminus. |
| Post Translational Modifications | The identity of the C-linked hexose on the WXXW motif has not been determined. It is probably mannose. On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-453 is required for PTPN6 interaction, Tyr-425 for PTPN11. Tyr-425 is also required for SOCS3 binding, but Tyr-453/Tyr-455 motif is the preferred binding site. Ubiquitinated by NOSIP.appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells. Ubiquitination at Lys-280 mediates receptor internalization, whereas ubiquitination at Lys-452 promotes trafficking of activated receptors to the lysosomes for degradation. |
| Function | Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase. |
| Protein Name | Erythropoietin ReceptorEpo-R |
| Database Links | Reactome: R-MMU-9006335Reactome: -MMU-9027276Reactome: -MMU-9027284 |
| Cellular Localisation | Isoform Epor-F: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Epor-S: Secreted |
| Alternative Protein Names | Erythropoietin Receptor proteinEpo-R proteinEpor protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
