Human Erythropoietin R/EPO-R protein (Recombinant) (C-hFc & His) (STJP002567)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Erythropoietin R/EPO-R Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala25-Pro250) of human EPO Receptor/EPOR (Accession #NP_000112.1) fused with an Fc, 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Erythropoietin R/EPO-R-C-hFc & His protein was developed in hek293 cells using the region Ala25-Pro250. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, 0.1mM EDTA and 0.1% CHAPS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Human EPO Protein at 2 Mu g/mL (100 Mu L/well) can bind EPOR with a linear range of 0.24-12.02 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | EPOR |
| Gene ID | 2057 |
| Uniprot ID | EPOR_HUMAN |
| Immunogen | Recombinant Human Erythropoietin R/EPO-R Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala25-Pro250) of human EPO Receptor/EPOR (Accession #NP_000112.1) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Region | Ala25-Pro250 |
| Immunogen Sequence | APPPNLPDPKFESKAALLAA RGPEELLCFTERLEDLVCFW EEAASAGVGPGNYSFSYQLE DEPWKLCRLHQAPTARGAVR FWCSLPTADTSSFVPLELRV TAASGAPRYHRVIHINEVVL LDAPVGLVARLADESGHVVL RWLPPPETPMTSHIRYEVDV SAGNGAGSVQRVEILEGRTE CVLSNLRGRTRYTFAVRARM AEPSFGGFWSAWSEPVSLLT PSDLDP |
Additional Info
| Post Translational Modifications | On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site. Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome. Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of erythropoietin-mediated signaling pathway. Ubiquitination at Lys-281 mediates receptor internalization, whereas ubiquitination at Lys-453 promotes trafficking of activated receptors to the lysosomes for degradation. Ubiquitinated by NOSIP.appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells. |
| Function | Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase. Isoform EPOR-T: Acts as a dominant-negative receptor of EPOR-mediated signaling. |
| Protein Name | Erythropoietin ReceptorEpo-R |
| Database Links | Reactome: R-HSA-9006335Reactome: R-HSA-9027276Reactome: R-HSA-9027277Reactome: R-HSA-9027283Reactome: R-HSA-9027284 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Epor-S: SecretedSecreted And Located To The Cell Surface |
| Alternative Protein Names | Erythropoietin Receptor proteinEpo-R proteinEPOR protein |
Information sourced from Uniprot.org