| Host: |
HEK293 cells |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse EphA2/ECK-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Gene Symbol: |
Epha2 |
| Gene ID: |
13836 |
| Uniprot ID: |
EPHA2_MOUSE |
| Immunogen: |
Recombinant Mouse EphA2/ECK Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys26-Asn535) of mouse EphA2/Sek2 (Accession #NP_034269.2) fused with a 6×His tag at the C-terminus. |
| Post Translational Modifications | Autophosphorylates. Phosphorylated at Ser-898 by PKB.serum-induced phosphorylation which targets EPHA2 to the cell leading edge and stimulates cell migration. Phosphorylation by PKB is inhibited by EFNA1-activated EPHA2 which regulates PKB activity via a reciprocal regulatory loop. Phosphorylated on tyrosine upon binding and activation by EFNA1. Phosphorylated residues Tyr-589 and Tyr-595 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-736 and Tyr-931 are required for binding PI3-kinase p85 subunit (PIK3R1, PIK3R2 or PIK3R3). These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and cell migration. Dephosphorylation of Tyr-931 by PTPRF prevents the interaction of EPHA2 with NCK1. Phosphorylated at Ser-898 in response to TNF by RPS6KA1 and RPS6KA3.RPS6KA-EPHA2 signaling pathway controls cell migration. Phosphorylated at Ser-898 by PKA.blocks cell retraction induced by EPHA2 kinase activity. Dephosphorylated by ACP1. Ubiquitinated by CHIP/STUB1. Ubiquitination is regulated by the HSP90 chaperone and regulates the receptor stability and activity through proteasomal degradation. ANKS1A prevents ubiquitination and degradation. |
| Function | Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis. |
| Protein Name | Ephrin Type-A Receptor 2Epithelial Cell KinaseTyrosine-Protein Kinase Receptor EckTyrosine-Protein Kinase Receptor Mpk-5Tyrosine-Protein Kinase Receptor Sek-2 |
| Database Links | Reactome: R-MMU-2682334Reactome: -MMU-3928663Reactome: -MMU-3928665Reactome: -MMU-9013149Reactome: -MMU-9013404Reactome: -MMU-9013408Reactome: -MMU-9013420Reactome: -MMU-9013423Reactome: -MMU-9013424Reactome: -MMU-9696264Reactome: -MMU-9696270Reactome: -MMU-9696273 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionRuffle MembraneLamellipodium MembraneCell JunctionFocal AdhesionPresent At Regions Of Cell-Cell Contacts But Also At The Leading Edge Of Migrating CellsRelocates From The Plasma Membrane To The Cytoplasmic And Perinuclear Regions In Cancer Cells |
| Alternative Protein Names | Ephrin Type-A Receptor 2 proteinEpithelial Cell Kinase proteinTyrosine-Protein Kinase Receptor Eck proteinTyrosine-Protein Kinase Receptor Mpk-5 proteinTyrosine-Protein Kinase Receptor Sek-2 proteinEpha2 proteinEck proteinMyk2 proteinSek2 protein |
Information sourced from Uniprot.org
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