This DNMT1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates or other biological fluids..
Applications
ELISA
Reactivity
Mouse
Sensitivity
0.053ng/mL
Detection Limit
0.156-10ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Isoform 1 is expressed in embryonic stem cells and in somatic tissues. Isoform 2 is expressed in oocytes, preimplantation embryos, testis and in skeletal muscle during myogenesis.
Post Translational Modifications
Sumoylated.sumoylation increases activity. Phosphorylation at Ser-146 by CK1 reduces DNA-binding activity. Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1352 and Lys-1418 by SIRT1 increases methyltransferase activity. Phosphorylation of Ser-152 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-140 by AKT1 prevents methylation by SETD7 thereby increasing DNMT1 stability. Methylation at Lys-139 by SETD7 is necessary for the regulation of DNMT1 proteasomal degradation. Ubiquitinated by UHRF1.interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.
Function
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth.
NucleusCytoplasmIt Is Nucleoplasmic Through Most Of The Cell Cycle And Associates With Replication Foci During S-PhaseIn Germ CellsSpermatogoniaPreleptotene And Leptotene Spermatocytes All Express High Levels Of Nuclear ProteinWhile The Protein Is Not Detected In Pachytene SpermatocytesDespite The Fact They Expressed High Levels Of MrnaIn FemalesThe Protein Is Not Detected In Non-Growing OocytesIn Contrast To The Growing OocytesDuring The GrowingThe Protein Is No Longer Detectable In Nuclei But Accumulates To Very High Levels First Throughout The CytoplasmAt The Time Of OvulationAll The Protein Is Cytoplasmic And Is Actively Associated With The Oocyte CortexAfter FecondationIn The Preimplantation EmbryoThe Protein Remains Cytoplasmic And After ImplantationIt Is Exclusively Nuclear In All Tissue TypesIsoform 2 Is Sequestered In The Cytoplasm Of Maturing Oocytes And Of Preimplantation EmbryosExcept For The 8-Cell StageWhile Isoform 1 Is Exclusively Nuclear
