| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.08ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description : | This DLD Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | Dld |
| Gene ID: | 13382 |
| Uniprot ID: | DLDH_MOUSE |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Expressed in liver (at protein level). |
| Post Translational Modifications | Tyrosine phosphorylated. |
| Function | Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction. |
| Protein Name | Dihydrolipoyl Dehydrogenase - MitochondrialDihydrolipoamide Dehydrogenase |
| Database Links | Reactome: R-MMU-204174Reactome: -MMU-5362517Reactome: -MMU-6783984Reactome: -MMU-70895Reactome: -MMU-9837999Reactome: -MMU-9853506Reactome: -MMU-9858328Reactome: -MMU-9859138Reactome: -MMU-9861559 |
| Cellular Localisation | Mitochondrion MatrixNucleusCell ProjectionCiliumFlagellumCytoplasmic VesicleSecretory VesicleAcrosomeMainly Localizes In The MitochondrionA Small Fraction Localizes To The NucleusWhere The 2-Oxoglutarate Dehydrogenase Complex Is Required For Histone Succinylation |
| Alternative ELISA Names | Dihydrolipoyl Dehydrogenase - Mitochondrial ELISA kitDihydrolipoamide Dehydrogenase ELISA kitDld ELISA kit |
| output |
Information sourced from Uniprot.org
