| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Supplied as a 0.22 Mu m filtered solution in 20mM Tris, 500mM NaCl, 10% Glycerol, pH 8.0. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA.Immobilized Mouse DKK-1 at 5 Mu g/mL (100 Mu L/well) can bind Human LRP-5 with a linear range of 0.001-0.623 Mu g/mL. |
| Gene Symbol: | Dkk1 |
| Gene ID: | 13380 |
| Uniprot ID: | DKK1_MOUSE |
| Immunogen Region: | Thr32-His272 |
| Immunogen: | Recombinant Mouse Dkk-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr32-His272) of mouse DKK-1 (Accession #NP_034181.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | TLNSVLINSNAIKNLPPPLG GAGGQPGSAVSVAPGVLYEG GNKYQTLDNYQPYPCAEDEE CGSDEYCSSPSRGAAGVGGV QICLACRKRRKRCMRHAMCC PGNYCKNGICMPSDHSHFPR GEIEESIIENLGNDHNAAAG DGYPRRTTLTSKIYHTKGQE GSVCLRSSDCAAGLCCARHF WSKICKPVLKEGQVCTKHKR KGSHGLEIFQRCYCGEGLAC RIQKDHHQASNSSRLHTCQ |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity. Plays a role in limb development.attenuates Wnt signaling in the developing limb to allow normal limb patterning. |
| Protein Name | Dickkopf-Related Protein 1Dickkopf-1Dkk-1Mdkk-1 |
| Database Links | Reactome: R-MMU-3772470 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Dickkopf-Related Protein 1 proteinDickkopf-1 proteinDkk-1 proteinMdkk-1 proteinDkk1 protein |
Information sourced from Uniprot.org
