Mouse DDAH2 (N (G) , N (G)-Dimethylarginine Dimethylaminohydrolase 2) Sandwich ELISA Kit Ready-To-Use Kit (STJE0014759)
SPECIFICATIONS
General Information
| Short Description | This DDAH2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma, tissue homogenates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Mouse |
| Sensitivity | 0.108ng/mL |
| Detection Limit | 0.312-20ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | Ddah2 |
| Gene ID | 51793 |
| Uniprot ID | DDAH2_MOUSE |
| Immunogen Region | Ready-To-Use |
| Sample Type | serum, plasma, tissue homogenates or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed at high levels in kidney and lung (at protein level). Expressed in islets of Langerhans. |
| Post Translational Modifications | Phosphorylated by TBK1. Phosphorylation inhibits the translocation into the mitochondrion upon Sendai viral infection. |
| Function | Putative hydrolase with unknown substrate (Probable). Does not hydrolyze N(G),N(G)-dimethyl-L-arginine (ADMA) which acts as an inhibitor of NOS. In endothelial cells, induces expression of vascular endothelial growth factor (VEGF) via phosphorylation of the transcription factor SP1 by PKA in a process that is independent of NO and NO synthase. Similarly, enhances pancreatic insulin secretion through SP1-mediated transcriptional up-regulation of secretagogin/SCGN, an insulin vesicle docking protein. Upon viral infection, relocates to mitochondria where it promotes mitochondrial fission through activation of DNM1L leading to the inhibition of innate response activation mediated by MAVS. |
| Protein Name | Putative Hydrolase Ddah2DdahiiInactive N(G -N(G-Dimethylarginine Dimethylaminohydrolase 2Ddah-2Inactive Dimethylarginine Dimethylaminohydrolase 2 |
| Database Links | |
| Cellular Localisation | CytoplasmMitochondrionTranslocates From Cytosol To Mitochondrion Upon Il1b Stimulation In Chondrocytes(Microbial Infection) Translocates To The Mitochondrion Upon Sendai Viral Infection |
| Alternative ELISA Names | Putative Hydrolase Ddah2 ELISA kitDdahii ELISA kitInactive N(G -N(G-Dimethylarginine Dimethylaminohydrolase 2 ELISA kitDdah-2 ELISA kitInactive Dimethylarginine Dimethylaminohydrolase 2 ELISA kitDdah2 ELISA kit |
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Information sourced from Uniprot.org