Mouse CYP27B1 (Cytochrome P450 27B1) Sandwich ELISA Kit Ready-To-Use Kit (STJE0014692)
SPECIFICATIONS
General Information
| Short Description | This CYP27B1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates, cell lysates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Mouse |
| Sensitivity | 0.214ng/mL |
| Detection Limit | 0.625-40ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | Cyp27b1 |
| Gene ID | 13115 |
| Uniprot ID | CP27B_MOUSE |
| Immunogen Region | Ready-To-Use |
| Sample Type | tissue homogenates, cell lysates or other biological fluids. |
Additional Info
| Tissue Specificity | Kidney. |
| Post Translational Modifications | |
| Function | A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1-alpha position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR). Has 1-alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin. |
| Protein Name | 25-Hydroxyvitamin D-1 Alpha Hydroxylase - Mitochondrial25-Ohd-1 Alpha-Hydroxylase25-Hydroxyvitamin D(3 1-Alpha-HydroxylaseVd3 1a HydroxylaseCalcidiol 1-MonooxygenaseCytochrome P450 Subfamily Xxviib Polypeptide 1Cytochrome P450c1 AlphaCytochrome P450vd1-AlphaCytochrome P450 27b1 |
| Database Links | Reactome: R-MMU-196791Reactome: -MMU-211916 |
| Cellular Localisation | Mitochondrion Membrane |
| Alternative ELISA Names | 25-Hydroxyvitamin D-1 Alpha Hydroxylase - Mitochondrial ELISA kit25-Ohd-1 Alpha-Hydroxylase ELISA kit25-Hydroxyvitamin D(3 1-Alpha-Hydroxylase ELISA kitVd3 1a Hydroxylase ELISA kitCalcidiol 1-Monooxygenase ELISA kitCytochrome P450 Subfamily Xxviib Polypeptide 1 ELISA kitCytochrome P450c1 Alpha ELISA kitCytochrome P450vd1-Alpha ELISA kitCytochrome P450 27b1 ELISA kitCyp27b1 ELISA kitCyp27b ELISA kitCyp40 ELISA kit |
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Information sourced from Uniprot.org