| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: |
Cripto |
| Gene ID: |
21667 |
| Uniprot ID: |
TDGF1_MOUSE |
| Immunogen Region: |
Arg26-Gln150 |
| Immunogen: |
Recombinant Mouse Cripto/TDGF1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg26-Gln150 ) of Mouse Cripto/TDGF1 (Accession #NP_035692.2) fused with a His tag at the C-terminus. |
| Immunogen Sequence: |
QKVTSLTACLVDQSLRLDCR HENTSSSPIQYEFSLTRETK KHVLFGTVGVPEHTYRSRTN FTSKYNMKVLYLSAFTSKDE GTYTCALHHSGHSPPISSQN VTVLRDKLVKC |
| Tissue Specificity | Expressed at low level in specific organs of the adult animal such as spleen, heart, lung and brain. During gastrulation, expressed in the forming mesoderm. In later stages of the developing heart, expression is restricted to the truncus arteriosus. |
| Post Translational Modifications | The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, it is processed by GPI processing phospholipase A2 (Tmem8a), removing an acyl-chain at the sn-2 position of GPI and releasing CRIPTO as a lysophosphatidylinositol-bearing form, which is further cleaved by phospholipase D (Gpld1) into a soluble form. |
| Function | GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by Tmem8a modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells. Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. |
| Protein Name | Teratocarcinoma-Derived Growth FactorCripto Growth FactorEpidermal Growth Factor-Like Cripto Protein |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorSecretedReleased From The Cell Membrane By Gpi Cleavage |
| Alternative Protein Names | Teratocarcinoma-Derived Growth Factor proteinCripto Growth Factor proteinEpidermal Growth Factor-Like Cripto Protein proteinCripto proteinTdgf1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
