| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 6.9pg/mL |
| Detection Limit: | 15.6-1000pg/mL |
| Short Description: | This CKB Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | Ckb |
| Gene ID: | 12709 |
| Uniprot ID: | KCRB_MOUSE |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in hippocampus and corpus callosum (at protein level). |
| Post Translational Modifications | Ubiquitinated by the ECS(ASB9) complex, leading to its degradation by the proteasome. |
| Function | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work. |
| Protein Name | Creatine Kinase B-Type B-Ck Creatine Kinase B Chain Creatine Phosphokinase B-Type Cpk-B |
| Database Links | Reactome: R-MMU-71288 Reactome: -MMU-9696264 |
| Cellular Localisation | Cytoplasm Cytosol Mitochondrion Cell Membrane Localizes To The Mitochondria Of Thermogenic Fat Cells Via The Internal Mts-Like Signal (Imts-L) Region |
| Alternative ELISA Names | Creatine Kinase B-Type ELISA kit B-Ck ELISA kit Creatine Kinase B Chain ELISA kit Creatine Phosphokinase B-Type ELISA kit Cpk-B ELISA kit Ckb ELISA kit Ckbb ELISA kit |
| output |
Information sourced from Uniprot.org
