| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.053ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This CDK8 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | Cdk8 |
| Gene ID: | 264064 |
| Uniprot ID: | CDK8_MOUSE |
| Sample Type: | tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. |
| Protein Name | Cyclin-Dependent Kinase 8 Cell Division Protein Kinase 8 Mediator Complex Subunit Cdk8 Mediator Of Rna Polymerase Ii Transcription Subunit Cdk8 |
| Database Links | Reactome: R-MMU-2173796 |
| Cellular Localisation | Nucleus |
| Alternative ELISA Names | Cyclin-Dependent Kinase 8 ELISA kit Cell Division Protein Kinase 8 ELISA kit Mediator Complex Subunit Cdk8 ELISA kit Mediator Of Rna Polymerase Ii Transcription Subunit Cdk8 ELISA kit Cdk8 ELISA kit |
| output |
Information sourced from Uniprot.org
