| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse Cathepsin L-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Dilution Range: |
Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Gene Symbol: |
Ctsl |
| Gene ID: |
13039 |
| Uniprot ID: |
CATL1_MOUSE |
| Immunogen Region: |
THR18-VAL333 |
| Immunogen: |
Optimized DNA sequence encoding mature chain of mouse Cathepsin L (THR18-VAL333) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Expressed in thymus, kidney and liver. Expressed in thyroid epithelial cells. Expressed in cortical thymic epithelial cells. Expressed by antigen presenting cells (APCs) such as dendritic cells and macrophages. |
| Post Translational Modifications | During export along the endocytic pathway, pro-CTSL undergoes several proteolytic cleavages to generate the CTSL single-chain and two-chain mature forms, composed of a heavy chain linked to a light chain by disulfide bonds. Autocleavage.produces the single-chain CTSL after cleavage of the propeptide. The cleavage can be intermolecular. |
| Function | Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen. In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter. In thymus, regulates CD4(+) T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells. Also mediates invariant chain processing in cortical thymic epithelial cells. Major elastin-degrading enzyme at neutral pH. Accumulates as a mature and active enzyme in the extracellular space of antigen presenting cells (APCs) to regulate degradation of the extracellular matrix in the course of inflammation. Secreted form generates endostatin from COL18A1. Critical for cardiac morphology and function. Plays an important role in hair follicle morphogenesis and cycling, as well as epidermal differentiation. Required for maximal stimulation of steroidogenesis by TIMP1. |
| Protein Name | Procathepsin LCathepsin L1Major Excreted ProteinMepP39 Cysteine Proteinase Cleaved Into - Cathepsin L - Cathepsin L Heavy Chain - Cathepsin L Light Chain |
| Database Links | Reactome: R-MMU-1442490Reactome: -MMU-1679131Reactome: -MMU-2022090Reactome: -MMU-2132295Reactome: -MMU-8939242 |
| Cellular Localisation | LysosomeApical Cell MembranePeripheral Membrane ProteinExtracellular SideSecretedExtracellular SpaceCytoplasmic VesicleSecretory VesicleChromaffin GranuleLocalizes To The Apical Membrane Of Thyroid Epithelial CellsReleased At Extracellular Space By Activated Dendritic Cells And Macrophages |
| Alternative Protein Names | Procathepsin L proteinCathepsin L1 proteinMajor Excreted Protein proteinMep proteinP39 Cysteine Proteinase Cleaved Into - Cathepsin L - Cathepsin L Heavy Chain - Cathepsin L Light Chain proteinCtsl proteinCtsl1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
