Mouse CASP1/Caspase-1 protein (Recombinant) (N-His) (STJP011110)
SPECIFICATIONS
HostE.coli
ImmunogenMus musculus (Mouse)
General Information
| Short Description | Recombinant-Mouse CASP1/Caspase-1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | Casp1 |
| Gene ID | 12362 |
| Uniprot ID | CASP1_MOUSE |
| Immunogen | Mus musculus (Mouse) |
| Immunogen Region | Leu137-His402 |
Additional Info
| Post Translational Modifications | The two subunits are derived from the precursor sequence by an autocatalytic mechanism. Ubiquitinated via 'Lys-11'-linked polyubiquitination. Deubiquitinated by USP8. |
| Function | Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukin IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly. |
| Protein Name | Caspase-1Casp-1Interleukin-1 Beta ConvertaseIl-1bcInterleukin-1 Beta-Converting EnzymeIceIl-1 Beta-Converting EnzymeP45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10 |
| Database Links | Reactome: R-MMU-168638Reactome: -MMU-448706Reactome: -MMU-5620971 |
| Cellular Localisation | CytoplasmCell Membrane |
| Alternative Protein Names | Caspase-1 proteinCasp-1 proteinInterleukin-1 Beta Convertase proteinIl-1bc proteinInterleukin-1 Beta-Converting Enzyme proteinIce proteinIl-1 Beta-Converting Enzyme proteinP45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10 proteinCasp1 proteinIl1bc protein |
Information sourced from Uniprot.org