Mouse C5/Complement C5 protein (Recombinant-Active) (No tag) (STJP011809)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenMus musculus (Mouse)
General Information
| Short Description | Recombinant-Active-Mouse C5/Complement C5-No tag protein was developed from e.coli and has a target region of No tag. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured by its ability to induce N-acetyl-beta-D-glucosaminidase release from differentiated human histiocytic lymphoma cells (U937). The ED50 for this effect is 5-20 ng/mL. |
Target Information
| Gene Symbol | C5 |
| Gene ID | 15139 |
| Uniprot ID | CO5_MOUSE |
| Immunogen | Mus musculus (Mouse) |
| Immunogen Region | Asn679-Arg755 |
Additional Info
| Function | Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Activated downstream of classical, alternative, lectin and GZMK complement pathways. Complement C5b: Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex: C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9. During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending. C5a anaphylatoxin: Mediator of local inflammatory process released following cleavage by C5 convertase. Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. |
| Protein Name | Complement C5Hemolytic Complement Cleaved Into - Complement C5 Beta Chain - Complement C5 Alpha Chain - C5a Anaphylatoxin - Complement C5bComplement C5 Alpha' Chain |
| Database Links | Reactome: R-MMU-166665Reactome: -MMU-174577Reactome: -MMU-375276Reactome: -MMU-418594Reactome: -MMU-977606 |
| Cellular Localisation | SecretedComplement C5b: SecretedTarget Cell MembraneSecreted As Soluble ProteinInserts Into The Cell Membrane Of Target CellsC5a Anaphylatoxin: Secreted |
| Alternative Protein Names | Complement C5 proteinHemolytic Complement Cleaved Into - Complement C5 Beta Chain - Complement C5 Alpha Chain - C5a Anaphylatoxin - Complement C5b proteinComplement C5 Alpha' Chain proteinC5 proteinHc protein |
Information sourced from Uniprot.org