| Host: |
HEK293 cells |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse c-Kit ligand/SCF/KITLG-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: |
1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.5-1.0 ng/mL, corresponding to a specific activity of 1.0×106-2.0×106 units/mg.2. Measured by its binding ability in a functional ELISA.Immo |
| Gene Symbol: |
Kitlg |
| Gene ID: |
17311 |
| Uniprot ID: |
SCF_MOUSE |
| Immunogen Region: |
Lys26-Ala189 |
| Immunogen: |
Recombinant Mouse c-Kit ligand/SCF/KITLG Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Lys26-Ala189) of mouse SCF (Accession #NP_038626.1.) fused with a 6×His tag at the C-terminus. |
| Post Translational Modifications | A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain. |
| Function | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. |
| Protein Name | Kit LigandHematopoietic Growth Factor KlMast Cell Growth FactorMgfSteel FactorStem Cell FactorScfC-Kit Ligand Cleaved Into - Soluble Kit LigandSkitlg |
| Database Links | Reactome: R-MMU-1257604Reactome: -MMU-1433557Reactome: -MMU-1433559Reactome: -MMU-5673001Reactome: -MMU-6811558 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: CytoplasmCytoplasmCytoskeletonCell MembraneCell ProjectionLamellipodiumFilopodiumSoluble Kit Ligand: Secreted |
| Alternative Protein Names | Kit Ligand proteinHematopoietic Growth Factor Kl proteinMast Cell Growth Factor proteinMgf proteinSteel Factor proteinStem Cell Factor proteinScf proteinC-Kit Ligand Cleaved Into - Soluble Kit Ligand proteinSkitlg proteinKitlg proteinKitl proteinMgf proteinSl proteinSlf protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
