Mouse B7-H1/PD-L1/CD274 protein (Recombinant) (C-His) (STJP002718)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse B7-H1/PD-L1/CD274 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Thr238) of mouse PD-L1/B7-H1 (Accession #NP_068693) fused with a 6ΓHis tag at the C-terminus.
General Information
| Short Description | Recombinant-Mouse B7-H1/PD-L1/CD274-C-His protein was developed in hek293 cells using the region Met1-Thr238. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20Β°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse PD-L1 at 10 Mu g/mL (100 Mu L/well) can bind Recombinant Mouse PD-1 with a linear range of 1.5-5 Mu g/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | Cd274 |
| Gene ID | 60533 |
| Uniprot ID | PD1L1_MOUSE |
| Immunogen | Recombinant Mouse B7-H1/PD-L1/CD274 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Thr238) of mouse PD-L1/B7-H1 (Accession #NP_068693) fused with a 6ΓHis tag at the C-terminus. |
| Immunogen Region | Met1-Thr238 |
| Immunogen Sequence | MRIFAGIIFTACCHLLRAFT ITAPKDLYVVEYGSNVTMEC RFPVERELDLLALVVYWEKE DEQVIQFVAGEEDLKPQHSN FRGRASLPKDQLLKGNAALQ ITDVKLQDAGVYCCIISYGG ADYKRITLKVNAPYRKINQR ISVDPATSEHELICQAEGYP EAEVIWTNSDHQPVSGKRSV TTSRTEGMLLNVTSSLRVNA TANDVFYCTFWRSQPGQNHT AELIIPELPATHPPQNRT |
Additional Info
| Post Translational Modifications | Ubiquitinated.STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation. Ubiquitinated by MARCHF8.leading to degradation. Deubiquitinated by USP22.leading to stabilization. |
| Function | Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. |
| Protein Name | Programmed Cell Death 1 Ligand 1Pd-L1Pdcd1 Ligand 1Programmed Death Ligand 1B7 Homolog 1B7-H1Cd Antigen Cd274 |
| Database Links | Reactome: R-MMU-389948 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEarly Endosome MembraneRecycling Endosome Membrane |
| Alternative Protein Names | Programmed Cell Death 1 Ligand 1 proteinPd-L1 proteinPdcd1 Ligand 1 proteinProgrammed Death Ligand 1 proteinB7 Homolog 1 proteinB7-H1 proteinCd Antigen Cd274 proteinCd274 proteinB7h1 proteinPdcd1l1 proteinPdcd1lg1 proteinPdl1 protein |
Information sourced from Uniprot.org