| Host: |
HEK293 cells |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse B7-H1/PD-L1/CD274-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse PD-L1 at 10 Mu g/mL (100 Mu L/well) can bind Recombinant Mouse PD-1 with a linear range of 1.5-5 Mu g/mL. |
| Gene Symbol: |
Cd274 |
| Gene ID: |
60533 |
| Uniprot ID: |
PD1L1_MOUSE |
| Immunogen Region: |
Met1-Thr238 |
| Immunogen: |
Recombinant Mouse B7-H1/PD-L1/CD274 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Thr238) of mouse PD-L1/B7-H1 (Accession #NP_068693) fused with a 6×His tag at the C-terminus. |
| Post Translational Modifications | Ubiquitinated.STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation. Ubiquitinated by MARCHF8.leading to degradation. |
| Function | Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. |
| Protein Name | Programmed Cell Death 1 Ligand 1Pd-L1Pdcd1 Ligand 1Programmed Death Ligand 1B7 Homolog 1B7-H1Cd Antigen Cd274 |
| Database Links | Reactome: R-MMU-389948 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEarly Endosome MembraneRecycling Endosome Membrane |
| Alternative Protein Names | Programmed Cell Death 1 Ligand 1 proteinPd-L1 proteinPdcd1 Ligand 1 proteinProgrammed Death Ligand 1 proteinB7 Homolog 1 proteinB7-H1 proteinCd Antigen Cd274 proteinCd274 proteinB7h1 proteinPdcd1l1 proteinPdcd1lg1 proteinPdl1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
