| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse CD86 at 2 Mu g/mL (100 Mu L/well) can bind Recombinant Human CTLA-4 with a linear range of 8-35 ng/mL. |
| Gene Symbol: | Cd86 |
| Gene ID: | 12524 |
| Uniprot ID: | CD86_MOUSE |
| Immunogen Region: | Val24-Glu245 |
| Immunogen: | Recombinant Mouse B7-2/CD86 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val24-Glu245) of mouse CD86/B7-2 (Accession #NP_062261.3) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | VSVETQAYFNGTAYLPCPFT KAQNISLSELVVFWQDQQKL VLYEHYLGTEKLDSVNAKYL GRTSFDRNNWTLRLHNVQIK DMGSYDCFIQKKPPTGSIIL QQTLTELSVIANFSEPEIKL AQNVTGNSGINLTCTSKQGH PKPKKMYFLITNSTNEYGDN MQISQDNVTELFSISNSLSL SFPDGVWHMTVVCVLETESM KISSKPLNFTQEFPSPQTYW KE |
| Post Translational Modifications | Polyubiquitinated.which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. |
| Function | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. |
| Protein Name | T-Lymphocyte Activation Antigen Cd86Activation B7-2 AntigenEarly T-Cell Costimulatory Molecule 1Etc-1Cd Antigen Cd86 |
| Database Links | Reactome: R-MMU-1257604Reactome: -MMU-389356Reactome: -MMU-389357Reactome: -MMU-389359Reactome: -MMU-389513Reactome: -MMU-6811558 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | T-Lymphocyte Activation Antigen Cd86 proteinActivation B7-2 Antigen proteinEarly T-Cell Costimulatory Molecule 1 proteinEtc-1 proteinCd Antigen Cd86 proteinCd86 protein |
Information sourced from Uniprot.org
