| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse CD80 at 2 Mu g/mL (100 Mu L/well) can bind Recombinant Human CTLA-4 with a linear range of 16-66 ng/mL. |
| Gene Symbol: |
Cd80 |
| Gene ID: |
12519 |
| Uniprot ID: |
CD80_MOUSE |
| Immunogen: |
Recombinant Mouse B7-1/CD80 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val38-Lys245) of mouse B7-1/CD80 (Accession #NP_033985.3) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: |
VDEQLSKSVKDKVLLPCRYN SPHEDESEDRIYWQKHDKVV LSVIAGKLKVWPEYKNRTLY DNTTYSLIILGLVLSDRGTY SCVVQKKERGTYEVKHLALV KLSIKADFSTPNITESGNPS ADTKRITCFASGGFPKPRFS WLENGRELPGINTTISQDPE SELYTISSQLDFNTTRNHTI KCLIKYGDAHVSEDFTWEKP PEDPPDSK |
| Function | Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells. In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses. |
| Protein Name | T-Lymphocyte Activation Antigen Cd80Activation B7-1 AntigenB7Cd Antigen Cd80 |
| Database Links | Reactome: R-MMU-1257604Reactome: -MMU-389356Reactome: -MMU-389357Reactome: -MMU-389359Reactome: -MMU-389513Reactome: -MMU-6811558 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | T-Lymphocyte Activation Antigen Cd80 proteinActivation B7-1 Antigen proteinB7 proteinCd Antigen Cd80 proteinCd80 proteinB7 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
