| Applications: | CLIA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description : | This mouse ANXA5 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | Anxa5 |
| Gene ID: | 11747 |
| Uniprot ID: | ANXA5_MOUSE |
| Specificity: | This kit recognizes Mouse ANXA5 in samples. No significant cross-reactivity or interference between Mouse ANXA5 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
| Function | This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. |
| Protein Name | Annexin A5 Anchorin Cii Annexin V Annexin-5 Calphobindin I Cpb-I Endonexin Ii Lipocortin V Placental Anticoagulant Protein 4 Pp4 Placental Anticoagulant Protein I Pap-I Thromboplastin Inhibitor Vascular Anticoagulant-Alpha Vac-Alpha |
| Database Links | Reactome: R-MMU-114608 |
| Alternative CLIA Names | Annexin A5 CLIA kit Anchorin Cii CLIA kit Annexin V CLIA kit Annexin-5 CLIA kit Calphobindin I CLIA kit Cpb-I CLIA kit Endonexin Ii CLIA kit Lipocortin V CLIA kit Placental Anticoagulant Protein 4 CLIA kit Pp4 CLIA kit Placental Anticoagulant Protein I CLIA kit Pap-I CLIA kit Thromboplastin Inhibitor CLIA kit Vascular Anticoagulant-Alpha CLIA kit Vac-Alpha CLIA kit Anxa5 CLIA kit Anx5 CLIA kit |
| Specificity | This kit recognizes Mouse ANXA5 in samples. No significant cross-reactivity or interference between Mouse ANXA5 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 100-113 | 107 |
| EDTA plasma(n=8) | 94-107 | 100 |
| Cell culture media(n=8) | 96-110 | 102 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 91.90 | 310.66 | 684.49 | 84.23 | 313.63 | 741.16 |
| Standard deviation | 8.93 | 29.82 | 75.98 | 10.05 | 29.14 | 46.84 |
| CV (%) | 9.72 | 9.60 | 11.10 | 11.93 | 9.29 | 6.32 |