| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse Angiopoietin-1-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 Mu m filtered 20mM Sodium Phosphate, pH7.5, 200mM NaCl, 5% Trehalose. |
| Dilution Range: |
A quick spin of the vial followed by gently mixing in distilled water to prepare a stock solution concentration not less than 0.1 mg/mL. Avoid vigorous shaking or vortex |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for twelve months, addition of a carrier protein to your working aliquots (0.1% BSA or HSA) is recommended for long term storage Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Gene Symbol: |
Angpt1 |
| Gene ID: |
11600 |
| Uniprot ID: |
ANGP1_MOUSE |
| Immunogen Region: |
Mature chain |
| Immunogen: |
Optimized DNA sequence encoding mature chain of Mouse Angiopoietin-1 including an C-terminal His tag was expressed in HEK293 cells. NA |
| Function | Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. |
| Protein Name | Angiopoietin-1Ang-1 |
| Database Links | Reactome: R-MMU-210993Reactome: -MMU-5673001 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Angiopoietin-1 proteinAng-1 proteinAngpt1 proteinAgpt protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
