Mouse ADP/Acrp30 (Adiponectin) Antibody Pair (STJAP00065)

SPECIFICATIONS
ClonalityPolyclonal/Polyclonal
HostGoat/Goat
ConjugationUnconjugated/Biotin
ImmunogenCapture immunogen: Recombinant Mouse ADP/Acrp30 protein.
Detection Immunogen: Recombinant Mouse ADP/Acrp30 protein.
STJAP00065-50
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General Information

Short DescriptionThis ADP/Acrp30 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA.
ApplicationsELISA
HostGoat/Goat
ReactivityMouse
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

ClonalityPolyclonal/Polyclonal
ConjugationUnconjugated/Biotin
Concentration0.5 mg/mL
PurificationCapture Purification: Antigen Affinity.
Detection Purification: Antigen Affinity.
FormulationCapture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.
Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.
Storage InstructionStore at-20°C for the long term, and avoid freeze-thaw cycles.

Target Information

Gene SymbolAdipoq
Gene ID11450
Uniprot IDADIPO_MOUSE
ImmunogenCapture immunogen: Recombinant Mouse ADP/Acrp30 protein.
Detection Immunogen: Recombinant Mouse ADP/Acrp30 protein.

Additional Info

Post Translational Modifications HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagen-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes. O-glycosylated. Not N-glycosylated O-linked glycans on hydroxylysine residues consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. O-linked glycosylation in the N-terminal is disialylated with the structure Neu5Acalpha2->8Neu5Acalpha2->3Gal. Sialylated by alpha 2,8-sialyltransferase III. Succination of Cys-39 by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits polymerization and secretion of adiponectin. Adiponectin is a major target for succination in both adipocytes and adipose tissue of diabetic mice. It was proposed that succination of proteins is a biomarker of mitochondrial stress and accumulation of Krebs cycle intermediates in adipose tissue in diabetes and that succination of adiponectin may contribute to the decrease in plasma adiponectin in diabetes.
Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Protein Name Adiponectin
30 Kda Adipocyte Complement-Related Protein
Adipocyte Complement-Related 30 Kda Protein
Acrp30
Adipocyte - C1q And Collagen Domain-Containing Protein
Adipocyte-Specific Protein Adipoq
Database Links Reactome: R-MMU-163680
Cellular Localisation Secreted
Alternative Antibody Pair Names Adiponectin antibody pair
30 Kda Adipocyte Complement-Related Protein antibody pair
Adipocyte Complement-Related 30 Kda Protein antibody pair
Acrp30 antibody pair
Adipocyte - C1q And Collagen Domain-Containing Protein antibody pair
Adipocyte-Specific Protein Adipoq antibody pair
Adipoq antibody pair
Acdc antibody pair
Acrp30 antibody pair
Apm1 antibody pair

Information sourced from Uniprot.org

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