| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to hydrolyze the 5 NA-phosphate group from the substrate adenosine-5 NA-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is >24790 pmol/min/Mu g. |
| Gene Symbol: | Nt5e |
| Gene ID: | 23959 |
| Uniprot ID: | 5NTD_MOUSE |
| Immunogen: | Recombinant Mouse 5-Nucleotidase/CD73 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Trp29-Ser551) of mouse CD73/NT5E (Accession #NP_035981.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | WELTILHTNDVHSRLEQTSD DSTKCLNASLCVGGVARLFT KVQQIRKEEPNVLFLDAGDQ YQGTIWFTVYKGLEVAHFMN ILGYDAMALGNHEFDNGVEG LIDPLLRNVKFPILSANIKA RGPLAHQISGLFLPSKVLSV GGEVVGIVGYTSKETPFLSN PGTNLVFEDEISALQPEVDK LKTLNVNKIIALGHSGFEMD KLIAQKVRGVDIVVGGHSNT FLYTGNPPSKEVPAGKYPF |
| Function | Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside. Hydrolyzes IMP. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Although AMP is the preferred substrate can also hydrolyze UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN. |
| Protein Name | 5'-Nucleotidase5'-Nt 5'-DeoxynucleotidaseEcto-5'-NucleotidaseImp-Specific 5'-NucleotidaseThymidylate 5'-PhosphataseCd Antigen Cd73 |
| Database Links | Reactome: R-MMU-196807Reactome: -MMU-73621Reactome: -MMU-74259 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-Anchor |
| Alternative Protein Names | 5'-Nucleotidase protein5'-Nt protein5'-Deoxynucleotidase proteinEcto-5'-Nucleotidase proteinImp-Specific 5'-Nucleotidase proteinThymidylate 5'-Phosphatase proteinCd Antigen Cd73 proteinNt5e proteinNt5 proteinNte protein |
Information sourced from Uniprot.org