Host: |
HEK293 cells |
Reactivity: |
Mouse |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Recombinant-Mouse 5 NA-Nucleotidase/CD73-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
Immunoreactivity: |
Measured by its ability to hydrolyze the 5 NA-phosphate group from the substrate adenosine-5 NA-monophosphate (AMP). The orthophosphate product isMeasured by a Malachite Green Phosphate Detection Kit. The specific activity is >24790 pmol/min/Mu g. |
Gene Symbol: |
Nt5e |
Gene ID: |
23959 |
Uniprot ID: |
5NTD_MOUSE |
Immunogen: |
Recombinant Mouse 5-Nucleotidase/CD73 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Trp29-Ser551) of mouse CD73/NT5E (Accession #NP_035981.1) fused with a 6×His tag at the C-terminus. |
Function | Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside. Hydrolyzes IMP. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Although AMP is the preferred substrate can also hydrolyze UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN. |
Protein Name | 5'-Nucleotidase5'-Nt 5'-DeoxynucleotidaseEcto-5'-NucleotidaseImp-Specific 5'-NucleotidaseThymidylate 5'-PhosphataseCd Antigen Cd73 |
Database Links | Reactome: R-MMU-196807Reactome: -MMU-73621Reactome: -MMU-74259 |
Cellular Localisation | Cell MembraneLipid-AnchorGpi-Anchor |
Alternative Protein Names | 5'-Nucleotidase protein5'-Nt protein5'-Deoxynucleotidase proteinEcto-5'-Nucleotidase proteinImp-Specific 5'-Nucleotidase proteinThymidylate 5'-Phosphatase proteinCd Antigen Cd73 proteinNt5e proteinNt5 proteinNte protein |
Information sourced from Uniprot.org
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