LARP7 Blocking Peptide for STJ501578 is synthetically produced from the 300-350 sequence and is suitable for use in western blot applications.
Applications
Immunodepletion/Immunocompetition
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Formulation
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Synthetic peptide taken within amino acid region 300-350 on human LARP7 protein.
Immunogen Region
300-350
Specificity
This blocking peptide is recommended for use in combination with LARP7 antibody, STJ501578
Additional Info
Function
RNA-binding protein that specifically binds distinct small nuclear RNA (snRNAs) and regulates their processing and function. Specifically binds the 7SK snRNA (7SK RNA) and acts as a core component of the 7SK ribonucleoprotein (RNP) complex, thereby acting as a negative regulator of transcription elongation by RNA polymerase II. The 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC). LARP7 specifically binds to the highly conserved 3'-terminal U-rich stretch of 7SK RNA.on stimulation, remains associated with 7SK RNA, whereas P-TEFb is released from the complex. LARP7 also acts as a regulator of mRNA splicing fidelity by promoting U6 snRNA processing. Specifically binds U6 snRNAs and associates with a subset of box C/D RNP complexes: promotes U6 snRNA 2'-O-methylation by facilitating U6 snRNA loading into box C/D RNP complexes. U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity. Binds U6 snRNAs with a 5'-CAGGG-3' sequence motif. U6 snRNA processing is required for spermatogenesis.
Peptide Name
La-Related Protein 7La Ribonucleoprotein Domain Family Member 7Hlarp7P-Tefb-Interaction Protein For 7sk StabilityPip7s
Cellular Localisation
NucleusNucleoplasm
Alternative Peptide Names
La-Related Protein 7 proteinLa Ribonucleoprotein Domain Family Member 7 proteinHlarp7 proteinP-Tefb-Interaction Protein For 7sk Stability proteinPip7s proteinLARP7 proteinHDCMA18P protein
