KIF14 Blocking Peptide for STJ501537 is synthetically produced from the 850-960 sequence and is suitable for use in western blot applications.
Applications
Immunodepletion/Immunocompetition
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Formulation
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Synthetic peptide taken within amino acid region 850-960 on human KIF14 protein.
Immunogen Region
850-960
Specificity
This blocking peptide is recommended for use in combination with KIF14 antibody, STJ501537
Additional Info
Function
Microtubule motor protein that binds to microtubules with high affinity through each tubulin heterodimer and has an ATPase activity. Plays a role in many processes like cell division, cytokinesis and also in cell proliferation and apoptosis. During cytokinesis, targets to central spindle and midbody through its interaction with PRC1 and CIT respectively. Regulates cell growth through regulation of cell cycle progression and cytokinesis. During cell cycle progression acts through SCF-dependent proteasomal ubiquitin-dependent protein catabolic process which controls CDKN1B degradation, resulting in positive regulation of cyclins, including CCNE1, CCND1 and CCNB1. During late neurogenesis, regulates the cerebellar, cerebral cortex and olfactory bulb development through regulation of apoptosis, cell proliferation and cell division. Also is required for chromosome congression and alignment during mitotic cell cycle process. Regulates cell spreading, focal adhesion dynamics, and cell migration through its interaction with RADIL resulting in regulation of RAP1A-mediated inside-out integrin activation by tethering RADIL on microtubules.
NucleusCytoplasmCytoskeletonSpindleMidbodyNuclear Localization Observed During InterphaseNuclear Localization Triggered By Entry Into MitosisCytoplasmic In InterphaseCytoplasmic In Metaphase CellsFrom Prophase To MetaphaseAccumulates At The Developing Spindle Poles And Their Associated MicrotubulesDuring AnaphaseAccumulates At The Spindle MidzoneLocalization To The Central Spindle And Midbody During Anaphase Is Dependent Upon Prc1 And Cit PresenceIn Cells Ready To Undergo AbscissionConcentrates At The Contractile Ring
Alternative Peptide Names
Kinesin-Like Protein Kif14 proteinKIF14 proteinKIAA0042 protein
