| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
IRF8 Blocking Peptide for STJ501501 is synthetically produced from the 350-400 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Irf8 |
| Gene ID: |
15900 |
| Uniprot ID: |
IRF8_MOUSE |
| Immunogen Region: |
350-400 |
| Specificity: |
This blocking peptide is recommended for use in combination with IRF8 antibody, STJ501501 |
| Immunogen: |
Synthetic peptide taken within amino acid region 350-400 on mouse Interferon Regulatory Factor 8 protein. |
| Tissue Specificity | Expressed in bone marrow macrophages (at protein level). Mainly expressed in lymphoid tissues. Predominantly expressed in CD8(+)-expressing dendritic cells. |
| Post Translational Modifications | Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes. Ubiquitination leads to its degradation by the proteasome. Sumoylated with SUMO3. Desumoylated by SENP1. |
| Function | Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Can both act as a transcriptional activator or repressor. Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes. Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection. Positively regulates macroautophagy in dendritic cells. Acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1. |
| Peptide Name | Interferon Regulatory Factor 8Irf-8Interferon Consensus Sequence-Binding ProteinIcsbp |
| Cellular Localisation | NucleusCytoplasmIn Resting MacrophagesLocalizes In The CytoplasmTranslocated In The Nucleus Upon Ifn-Gamma Induction |
| Alternative Peptide Names | Interferon Regulatory Factor 8 proteinIrf-8 proteinInterferon Consensus Sequence-Binding Protein proteinIcsbp proteinIrf8 proteinIcsbp proteinIcsbp1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
