| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
IFITM3 Blocking Peptide for STJ501405 is synthetically produced from the 1-50 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
IFITM3 |
| Gene ID: |
10410 |
| Uniprot ID: |
IFM3_HUMAN |
| Immunogen Region: |
1-50 |
| Specificity: |
This blocking peptide is recommended for use in combination with IFITM3 antibody, STJ501405 |
| Immunogen: |
Synthetic peptide taken within amino acid region 1-50 on human Interferon-induced transmembrane protein 3. |
| Post Translational Modifications | Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus and hepatitis C virus (HCV). Has no effect on anti-SARS-CoV-2 activity. Not glycosylated. Polyubiquitinated with both 'Lys-48' and 'Lys-63' linkages. Ubiquitination negatively regulates antiviral activity. Lys-24 is the most prevalent ubiquitination site. Phosphorylation at Tyr-20 is required for endosomal and lysosomal location. |
| Function | IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis. Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell, by letting them go out with a cholesterol depleted membrane. Active against multiple viruses, including influenza A virus, SARS coronaviruses (SARS-CoV and SARS-CoV-2), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1), hepatitis C virus (HCV) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV and SARS-CoV-2 S protein-mediated viral entry and VSV G protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state. In hepatocytes, IFITM proteins act in a coordinated manner to restrict HCV infection by targeting the endocytosed HCV virion for lysosomal degradation. IFITM2 and IFITM3 display anti-HCV activity that may complement the anti-HCV activity of IFITM1 by inhibiting the late stages of HCV entry, possibly in a coordinated manner by trapping the virion in the endosomal pathway and targeting it for degradation at the lysosome. Exerts opposing activities on SARS-CoV-2, including amphipathicity-dependent restriction of virus at endosomes and amphipathicity-independent enhancement of infection at the plasma membrane. |
| Peptide Name | Interferon-Induced Transmembrane Protein 3Dispanin Subfamily A Member 2bDspa2bInterferon-Inducible Protein 1-8u |
| Database Links | Reactome: R-HSA-909733 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinLate Endosome MembraneEarly Endosome MembraneLysosome MembraneCytoplasmPerinuclear RegionCo-Localizes With Bri3 Isoform 1 At The Perinuclear Region |
| Alternative Peptide Names | Interferon-Induced Transmembrane Protein 3 proteinDispanin Subfamily A Member 2b proteinDspa2b proteinInterferon-Inducible Protein 1-8u proteinIFITM3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
