HYPE Positive Control for STJ506116 is synthetically produced from the sequence and is suitable for use in western blot applications.
Applications
WB
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Dilution Range
WB: 1:500-1:5, 000
Formulation
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
This is positive control is recommended for use in combination with HYPE antibody STJ506116.
Additional Info
Tissue Specificity
Ubiquitous.
Post Translational Modifications
Auto-AMPylated in vitro.it is unclear whether auto-AMPylation is relevant in vivo. N-glycosylated.predominantly glycosylated at Asn-275.
Function
Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP. In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates.
Peptide Name
Protein Adenylyltransferase FicdAmpylator FicdDe-Ampylase FicdFic Domain-Containing ProteinHuntingtin Yeast Partner EHuntingtin-Interacting Protein 13Hip-13Huntingtin-Interacting Protein E
Cellular Localisation
Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane Protein
Alternative Peptide Names
Protein Adenylyltransferase Ficd proteinAmpylator Ficd proteinDe-Ampylase Ficd proteinFic Domain-Containing Protein proteinHuntingtin Yeast Partner E proteinHuntingtin-Interacting Protein 13 proteinHip-13 proteinHuntingtin-Interacting Protein E proteinFICD proteinHIP13 proteinHYPE proteinUNQ3041 proteinPRO9857 protein
