HYPE Blocking Peptide for STJ506116 is synthetically produced from the 400-450 sequence and is suitable for use in western blot applications.
Applications
Immunodepletion/Immunocompetition
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Formulation
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Synthetic peptide corresponding to amino acids within region 400-450 on mouse Protein adenylyltransferase FICD.
Immunogen Region
400-450
Specificity
This blocking peptide is recommended for use in combination with HYPE antibody, STJ506116
Additional Info
Tissue Specificity
Ubiquitous.
Post Translational Modifications
Auto-AMPylated in vitro.it is unclear whether auto-AMPylation is relevant in vivo. N-glycosylated.predominantly glycosylated at Asn-275.
Function
Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP. In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates.
Peptide Name
Protein Adenylyltransferase FicdAmpylator FicdDe-Ampylase FicdFic Domain-Containing ProteinHuntingtin Yeast Partner EHuntingtin-Interacting Protein 13Hip-13Huntingtin-Interacting Protein E
Cellular Localisation
Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane Protein
Alternative Peptide Names
Protein Adenylyltransferase Ficd proteinAmpylator Ficd proteinDe-Ampylase Ficd proteinFic Domain-Containing Protein proteinHuntingtin Yeast Partner E proteinHuntingtin-Interacting Protein 13 proteinHip-13 proteinHuntingtin-Interacting Protein E proteinFICD proteinHIP13 proteinHYPE proteinUNQ3041 proteinPRO9857 protein
