| Host: | E.coli |
| Applications: | ELISA/Immunogen/SDS-PAGE/WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human WAS-N-GST & C-His protein was developed from e.coli and has a target region of N-GST & C-His. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Dilution Range: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | WAS |
| Gene ID: | 7454 |
| Uniprot ID: | WASP_HUMAN |
| Immunogen Region: | Gly40-Glu131 |
| Immunogen: | Homo sapiens (Human) |
| Post Translational Modifications | Phosphorylated at Tyr-291 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-291 enhances WAS activity in promoting actin polymerization and filopodia formation. |
| Function | Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). |
| Protein Name | Actin Nucleation-Promoting Factor WasWiskott-Aldrich Syndrome ProteinWasp |
| Database Links | Reactome: R-HSA-202433Reactome: R-HSA-2029482Reactome: R-HSA-5663213Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013409Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCytoskeletonNucleus |
| Alternative Protein Names | Actin Nucleation-Promoting Factor Was proteinWiskott-Aldrich Syndrome Protein proteinWasp proteinWAS proteinIMD2 protein |
Information sourced from Uniprot.org