Post Translational Modifications | VEGF-B186 is O-glycosylated. |
Function | Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. |
Protein Name | Vascular Endothelial Growth Factor BVegf-BVegf-Related FactorVrf |
Database Links | Reactome: R-HSA-114608Reactome: R-HSA-194313Reactome: R-HSA-195399 |
Cellular Localisation | SecretedSecreted But Remains Associated To Cells Or To The Extracellular Matrix Unless Released By Heparin |
Alternative ELISA Names | Vascular Endothelial Growth Factor B ELISA kitVegf-B ELISA kitVegf-Related Factor ELISA kitVrf ELISA kitVEGFB ELISA kitVRF ELISA kit |
Specificity | This kit recognizes Human VEGF-B in samples. No significant cross-reactivity or interference between Human VEGF-B and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 87-101 | 94 |
EDTA plasma (n=8) | 93-105 | 98 |
Cell culture media (n=8) | 92-107 | 98 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 51.07 | 102.94 | 400.36 | 51.34 | 106.20 | 365.76 |
Standard deviation | 2.75 | 4.67 | 13.05 | 3.14 | 4.59 | 16.06 |
CV (%) | 5.38 | 4.54 | 3.26 | 6.12 | 4.32 | 4.39 |