Human VEGF Receptor 2 (VEGF Receptor 2) Antibody Pair (STJAP00833)
General Information
| Short Description | This VEGF Receptor 2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R25-6I-6/R84-5M-1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | KDR |
| Gene ID | 3791 |
| Uniprot ID | VGFR2_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Detected in cornea (at protein level). Widely expressed. |
| Post Translational Modifications | N-glycosylated. Ubiquitinated. Tyrosine phosphorylation of the receptor promotes its poly-ubiquitination, leading to its degradation via the proteasome or lysosomal proteases. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-951 is important for interaction with SH2D2A/TSAD and VEGFA-mediated reorganization of the actin cytoskeleton. Phosphorylation at Tyr-1175 is important for interaction with PLCG1 and SHB. Phosphorylation at Tyr-1214 is important for interaction with NCK1 and FYN. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. The inhibitory disulfide bond between Cys-1024 and Cys-1045 may serve as a specific molecular switch for H(2)S-induced modification that regulates KDR/VEGFR2 function. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC. |
| Protein Name | Vascular Endothelial Growth Factor Receptor 2Vegfr-2Fetal Liver Kinase 1Flk-1Kinase Insert Domain ReceptorKdrProtein-Tyrosine Kinase Receptor Flk-1Cd Antigen Cd309 |
| Database Links | Reactome: R-HSA-194306Reactome: R-HSA-195399Reactome: R-HSA-216083Reactome: R-HSA-4420097Reactome: R-HSA-5218921Reactome: R-HSA-9673768Reactome: R-HSA-9856530 |
| Cellular Localisation | Cell JunctionEndoplasmic ReticulumCell MembraneLocalized With Rap1a At Cell-Cell JunctionsColocalizes With Ern1 And Xbp1 In The Endoplasmic Reticulum In Endothelial Cells In A Vascular Endothelial Growth Factor (Vegf)-Dependent MannerIsoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmNucleusCytoplasmic VesicleEarly EndosomeDetected On Caveolae-Enriched Lipid Rafts At The Cell SurfaceIs Recycled From The Plasma Membrane To Endosomes And Back AgainPhosphorylation Triggered By Vegfa Binding Promotes Internalization And Subsequent DegradationVegfa Binding Triggers Internalization And Translocation To The NucleusIsoform 2: SecretedIsoform 3: Secreted |
| Alternative Antibody Pair Names | Vascular Endothelial Growth Factor Receptor 2 antibody pairVegfr-2 antibody pairFetal Liver Kinase 1 antibody pairFlk-1 antibody pairKinase Insert Domain Receptor antibody pairKdr antibody pairProtein-Tyrosine Kinase Receptor Flk-1 antibody pairCd Antigen Cd309 antibody pairKDR antibody pairFLK1 antibody pairVEGFR2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human VEGF Receptor 2 (VEGF Receptor 2) Antibody Pair (STJAP00833)
This antibody pair is designed to detect human VEGF Receptor 2 (UniProt P35968). Reactivity has been confirmed for human samples only.
This antibody pair is validated for use in ELISA. It has not been tested or validated for other assay formats such as lateral flow or multiplex immunoassays.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL, formulated in 0.01M PBS at pH 7.2, and purified by affinity chromatography. Clone IDs are R25-6I-6 and R84-5M-1 for the two components of the pair.
Optimal working concentrations are determined by the end user and are not pre-defined in the product specifications. Titration experiments are recommended to establish appropriate dilutions for each specific experimental setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to preserve antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.