Human VDR (Vitamin D3 receptor) Sandwich ELISA Kit (STJE0003409)

Name: Human VDR (Vitamin D3 receptor) Sandwich ELISA Kit (STJE0003409)
SKU: STJE0003409
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0003409

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Short Description	This VDR Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.38ng/mL
Detection Limit	0.63~40ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	VDR
Gene ID	7421
Uniprot ID	VDR_HUMAN
Specificity	This kit recognizes Human VDR in samples. No significant cross-reactivity or interference between Human VDR and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Plays a central role in calcium homeostasis. Also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites.
Protein Name	Vitamin D3 Receptor Vdr 1 -25-Dihydroxyvitamin D3 Receptor Nuclear Receptor Subfamily 1 Group I Member 1
Database Links	Reactome: R-HSA-196791 Reactome: R-HSA-383280 Reactome: R-HSA-4090294
Cellular Localisation	Nucleus Cytoplasm Localizes Mainly To The Nucleus Translocated Into The Nucleus Via Both Ligand-Dependent And Ligand-Independent Pathways Ligand-Independent Nuclear Translocation Is Mediated By Ipo4
Alternative ELISA Names	Vitamin D3 Receptor ELISA kit Vdr ELISA kit 1 -25-Dihydroxyvitamin D3 Receptor ELISA kit Nuclear Receptor Subfamily 1 Group I Member 1 ELISA kit VDR ELISA kit NR1I1 ELISA kit
Specificity	This kit recognizes Human VDR in samples. No significant cross-reactivity or interference between Human VDR and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	1.85	5.89	18.83	1.82	6.22	17.53
Standard deviation	0.10	0.35	0.69	0.10	0.35	0.82
CV (%)	5.41	5.94	3.66	5.49	5.63	4.68